Genotyping

Genotyping:

  1. Before starting your project, please ensure that your dye sets are compatible with our Applied Biosystems 3730XL DNA Analyzers.
  2. The dye set best suited for our 3730XL is DS-33, which includes these dyes: 6-FAM, VIC, NED, PET, and LIZ (LIZ Size Standard). Please call us if you must use other dye sets.
  3. To submit your samples, you will need to use a 96 well half skirted or semi skirted PCR plate.  

    We prefer samples to be submitted in a specific type of plate:

Plasmid and Genomic DNA Prep

Plasmid Prep: 96well block format only.  $80/block for UC labs

This service utilizes magnetic bead technology for high-throughput purification of plasmid DNA from E. coli. It works for both high and low copy plasmids.

Contact us the day before submitting your cultures to confirm that someone will be available to process your samples. We cannot accept culture blocks on the weekend.

To take advantage of this, you will need:

PCR Clean Up

PCR Clean Up

We use magnetic SPRI bead technology to purify PCR products >100 bp, and elute in 40μl H2O (enough for 6-7 sequencing reactions) 

Submit PCR products in 10μl total volume according to these guidelines:

1000bp     80~160ng

600bp       50~100ng

400bp       30~60ng

200bp       15~30ng

150L New Brunswick BioFlo Fermentor

150L New Brunswick BioFlo Fermentor is an OPEN ACCESS INSTRUMENT for AUTHORIZED USERS and there is NO USER FEE. 

You need to have your own BUA and the prior experience with running of a large fermentor in order to use this instrument.

For more detail, please send an email to dnaseq@berkeley.edu or hitomi@berkeley.edu

Plasmid Prep

Plasmid Prep:

This service utilizes a magnetic bead technology for high-throughput purificatin fo plasmid DNA from E. Coli.   It works for both high and low copy plasmid.

You will need to contact us the day before submitting your cultures to confirm that someone will be available to process your samples.

To take advantage of this, you will need to submit:

PCR Clean Up

PCR Clean Up:

By Plate:  $36/plate

NOTE: We elute PCR products in 40ul of H2O.   Enough for 6~7 sequencing reactions.

1.  For PCR reactions that consist of a single product and PCR products over 100bp.

2.  Up to 95 samples (we need well A1 for control).

3.  Please indicate on your order form the volumes of your PCR reactions.

PCR Clean Up

PCR Clean Up:

By Plate:  $36/plate

NOTE: We elute PCR products in 40ul of H2O.   Enough for 6~7 sequencing reactions.

1.  For PCR reactions that consist of a single product and PCR products over 100bp.

2.  Up to 95 samples (we need well A1 for control).

3.  Please indicate on your order form the volumes of your PCR reactions.

Fragment Analysis

Fragment Analysis:

  1. Before you start your project, please make sure that you use the dye sets compatible with our Applied Biosystems 3730XL DNA Analyzers.
  2. The dye set best suited for our 3730XL is DS-33, which includes these dyes: 6-FAM, VIC, NED, PET,  and LIZ (LIZ Size Standard).  Please call us if you must use other dye sets.
  3. To submit your samples, you will need to use a 96 well half skirted or semi skirted PCR plate.  

    We prefer samples to be submitted in a specific type of plate:

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