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Departmaent of Molecular & Cell Biology

Sequencing Facility Main Page

Description of Services

How to Submit Samples

Contact Information

Frequently Asked Questions

Links to Related Sites

UC Berkeley DNA Sequencing Facility
303 Barker Hall #3202
Berkeley, California 94720-3202
dnaseq@berkeley.edu
(510)642-6383
Welcome to the UC Berkeley DNA Sequencing Facility.



What we do
We sequence DNA samples from University of California affiliated laboratories, government agencies and businesses. We also accept genotyping and fragment analysis projects. Call us to find out more.
 
What we Charge
UC laboratories: $5.00 per sample for samples in tubes (for LBNL labs add 3%) $7.65 per sample for off campus laboratories. For samples in 96 well plates the on campus charge is $313.50 per plate (for LBNL labs add 3%). Call us for more information before submitting sample in a plate.
 
How long does it take?
If you drop off your samples by 11:10am in room 237 Stanley, 11:30am in room 317 Barker, 11:30am in room 136 LSA or we receive them in the mail before 12 noon, we will send out results via e-mail by the following business day. Call us if you miss these drop off times. We may be able to accommodate your samples.

Results can sometimes be delayed by equipment problems. If you do not receive your results by e-mail or a notification of a delay in one working day do not hesitate to e-mail or call.
 
How to Submit Samples Click Here
 
Location
The DNA Sequencing Facility is in Barker Hall, Room 303. You can mail samples overnight without ice to:
UC Berkeley
DNA Sequencing Facility
303 Barker Hall #3202
Berkeley CA 94720-3202
We have four other drop off sites: 136 LSA (Life Sciences Addition ), 317 Barker Hall, or 237 Stanley Hall.
 
Need old results?
Unless you ask us not to, we archive all results. So if you lose your files, we can send them to you again. Our archive goes back to July 1996.
 
Changes and Updates sun-like ball image sun-like ball image sun-like ball image sun-like ball image sun-like ball image sun-like ball image sun-like ball image sun-like ball image

05/22/2007
New drop off site: 237 Stanley Hall. Pick up time: 11:10am

07/2006
We now have two Applied Biosystems 96 capillary 3730xl DNA Analyers.

06/05/2006
New drop off site: 136 LSA

12/28/2005
Our new Applied Biosystems 96-capillary 3730xl has arrived.

10/01/2004
We now charge $5.00 per sample for UC affiliated labs and $7.65 for non-UC affiliated labs.

09/27/2004
It is best if you add the primer. If you do not have a primer on hand and you do not have time to order it, you can pick up an aliquot for free or we can mail one to you. We have the following primers:
M13 Reverse (-24)(16-mer): 5'-AACAGCTATGACCATG-3'
M13 Forward (-21)(18-mer): 5'-TGTAAAACGACGGCCAGT-3'
T7(20-mer): 5'-TAATACGACTCACTATAGGG-3'
T7 Reverse(19-mer): 5'-GCTAGTTATTGCTCAGCGG-3'
T3(17-mer): 5'-ATTAACCCTCACTAAAG-3'
SP6(19-mer): 5'-GATTTAGGTGACACTATAG-3'
If you need us to add the primer,
put a large note in red on your order form, and your samples should be just 10uL total volume.

03/01/2004
We now have a new Applied Biosystems 48-capillary 3730 DNA Analyzer. This gives us a higher capacity and the ability to get results out faster with longer read lengths. We can now get read lengths of up to 1000 bases. The enhanced optical design provides a better signal-to-noise ratio that helps us give you high-quality data and the longest read lengths of any available system.

08/2001
Restrictions on sample names: We are now asking that your sample names be kept short. Your labels can use any letter, number or the following punctuation: -_(){}#.+. We ask that you write these labels on both the TOP of the tubes and on your order form.

If you would like to keep track of your samples with a longer label, write your own label on the side of your tube. A simple labeling system should be on TOP of the tubes, and it should match exactly what is on your order form.




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